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Violet ZnSe/ZnS as an Alternative to Green CdSe/ZnS in Nanocrystal−Fluorescent Protein FRET Systems

2009; American Chemical Society; Volume: 20; Issue: 6 Linguagem: Inglês

10.1021/bc9001085

1520-4812

Vitor Renaux Hering, Tanize Espírito Santo Faulin, Eduardo Rezende Triboni, Silvio D. Rodríguez, Delia L. Bernik, Robert I. Schumacher, Victor P. Mammana, Adelaide Faljoni‐Alário, Dulcinéia Saes Parra Abdalla, Gary A. P. Gibson, Mário J. Politi,

Advanced Biosensing Techniques and Applications

Fluorescent proteins from the green fluorescent protein family strongly interact with CdSe/ZnS and ZnSe/ZnS nanocrystals at neutral pH. Green emitting CdSe/ZnS nanocrystals and red emitting fluorescent protein dTomato constitute a 72% efficiency FRET system with the largest alteration of the overall photoluminescence profile, following complex formation, observed so far. The substitution of ZnSe/ZnS for CdSe/ZnS nanocrystals as energy donors enabled the use of a green fluorescent protein, GFP5, as energy acceptor. Violet emitting ZnSe/ZnS nanocrystals and green GFP5 constitute a system with 43% FRET efficiency and an unusually strong sensitized emission. ZnSe/ZnS-GFP5 provides a cadmium-free, high-contrast FRET system that covers only the high-energy part of the visible spectrum, leaving room for simultaneous use of the yellow and red color channels. Anisotropic fluorescence measurements confirmed the depolarization of GFP5 sensitized emission.