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Genetic heterogeneity in Prevotella intermedia , Prevotella nigrescens , Prevotella corporis and related species isolated from oral and nonoral sites

2000; Wiley; Volume: 15; Issue: 2 Linguagem: Inglês

10.1034/j.1399-302x.2000.150204.x

1399-302X

Michelle A. Pearce, Deirdre DeVine, Ronald Dixon, T. J. M. van Steenbergen,

Infective Endocarditis Diagnosis and Management

Prevotella intermedia (43 isolates), Prevotella nigrescens (55) and Prevotella corporis (8) from oral and nonoral sites were distinguished by species‐specific DNA fragments, after hybridization of DNA fragments with ribosomal RNA (ribotyping). Eight strains previously identified as P. intermedia did not have these specific fragments. P. nigrescens , P. intermedia and P. corporis formed separate clusters in dendrograms constructed using clustering with an unweighted pair group method with arithmetic averages of similarity values derived from ribotype patterns, with 10 subclusters in P. intermedia isolates and 26 in P. nigrescens . Nine groups of P. intermedia isolates and 6 of P. nigrescens shared identical patterns. Specific ribotypes or species were not associated with particular diseases when all isolates were analyzed. However, results from organisms isolated by one laboratory using consistent clinical reporting indicated that P. intermedia was associated with more severe forms of periodontitis and P. nigrescens with mild to moderate disease.