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Glucose and mannose diphosphate in the red blood cell

1968; Elsevier BV; Volume: 156; Issue: 2 Linguagem: Inglês

10.1016/0304-4165(68)90252-3

1872-8006

Grant R. Bartlett,

Pancreatic function and diabetes

1. Two compounds were isolated in highly purified from outdated blood bank human erythrocytes and were identified as α-d-glucose 1,6-diphosphate and α-d-mannose 1,6-diphosphate on the basis of the activation of phosphoglucomutase, the rate of acid hydrolysis, comparison of the properties of the monophosphaet derivatives with authentic glucose 6-phosphate and mannose 6-phosphate and comparison of the free sugar derivatives with known glucose and mannose. 2. The glucose and mannose diphosphates were found in concentrations between 0.1 and 0.2 μmole (phosphate) per ml in freshly collected human erythrocytes and in the same concentraion range in the red cells of rat, mouse, rabbit, horse, dog, cat, porpoise, sheep, beef, camel, guinea pig and pig. 3. On the basis of existing data, the concentration of glucose 1,6-diphosphate is much higher in mammalian red cells than in other kinds of tissue. 4. Mannose 1,6-diphosphate has not been reported previously to be a constituent of animal tissue. This compound may have been formed in the red cell from fructose 6-phosphate and glucose 1,6-diphosphate by the combined action of mannose 6-phosphate and glucose 6-phosphate isomerases and phosphoglucomutase. 5. It would appear that these relatively high concentrations of glucose and mannose diphosphates are unique characteristics of the mammalian red blood cell.