Direction of spreading (DOS): a simple method for measuring the hydrophobicity of bacterial lawns
1987; Elsevier BV; Volume: 6; Issue: 4 Linguagem: Inglês
10.1016/0167-7012(87)90050-9
ISSN1872-8359
Autores Tópico(s)Pharmaceutical and Antibiotic Environmental Impacts
ResumoA novel method is described here for the rapid evaluation of relative hydrophobicity values of bacterial lawns. This method, referred to as ‘Direction of Spreading’ (DOS), consists of recording the direction to which water drop spreads when introduced at the border between lawns and other surfaces. In order to standardized this new method, hydrophobicity values obtained by the DOS method were compared to those obtained by two existing methods: Contact angle and bacterial adhesion to hexadecane (BATH). Hydrophobicity values obtained by the DOS method were in good correlation to those measured by the contact method. However, in five out of twelve strains tested, here was a large difference mutant strains with decreased cell surface hydrophobicity (BATH) did not differ significantly from the parent strain with regard to colonical hydrophobicity (DOS). The DOS methods was applied to measure the increasing surface hydrophobicity of Streptomyces clavuligerus during development.
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