Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol
2002; Elsevier BV; Volume: 211; Issue: 1-4 Linguagem: Inglês
10.1016/s0044-8486(02)00003-0
ISSN1873-5622
AutoresJ. Głogowski, R. Kolman, M. Szczepkowski, Ákos Horváth, Béla Urbányi, Piotr Sieczyński, A. Rzemieniecki, Józef Domagała, W. Demianowicz, Radosław Kajetan Kowalski, Andrzej Ciereszko,
Tópico(s)Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
ResumoMilt obtained from three Siberian sturgeon males (Acipenser baeri, Brandt) were cryopreserved using three extenders: Tris–sucrose–KCl (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl, pH 8.0), Tris–NaCl (10 mM Tris, 25 mM NaCl, pH 8.5), and Tris–sucrose (20 mM Tris, 400 mM sucrose, pH 8.0) supplemented with 10% methanol. Semen was diluted 1:1 with appropriate extender and frozen in liquid nitrogen vapor. After fertilization with cryopreserved milt, hatching rates of 29.6±5.0%, 18.2±2.4%, and 6.0±3.0% were recorded for Tris–sucrose–KCl, Tris–NaCl, and Tris–sucrose extender, respectively. Rates for the first two extenders were similar to data of fresh semen obtained from two males(17.9% and 26.0% for male #1 and #2, respectively). Our results indicate that Tris–sucrose–KCl and Tris–NaCl are useful extenders and methanol is a useful cryoprotectant for cryopreservation of sturgeon semen.
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