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Localization of NADPH diaphorase/nitric oxide synthase and choline acetyltransferase in the spinal cord of the frog,Rana perezi

2000; Wiley; Volume: 419; Issue: 4 Linguagem: Inglês

10.1002/(sici)1096-9861(20000417)419

1096-9861

Margarita Muñoz, Oscar Marı́n, Agustı́n González,

Physiological and biochemical adaptations

Journal of Comparative NeurologyVolume 419, Issue 4 p. 451-470 Article Localization of NADPH diaphorase/nitric oxide synthase and choline acetyltransferase in the spinal cord of the frog, Rana perezi Margarita Muñoz, Margarita Muñoz Departamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, SpainSearch for more papers by this authorOscar Marín, Oscar Marín Departamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, Spain Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, Langley Porter Psychiatric Institute, University of California, San Francisco, California 94143-0984Search for more papers by this authorAgustín González, Corresponding Author Agustín González [email protected] Departamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 SpainSearch for more papers by this author Margarita Muñoz, Margarita Muñoz Departamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, SpainSearch for more papers by this authorOscar Marín, Oscar Marín Departamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, Spain Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, Langley Porter Psychiatric Institute, University of California, San Francisco, California 94143-0984Search for more papers by this authorAgustín González, Corresponding Author Agustín González [email protected] Departamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Biología Celular, Facultad de Biología, Universidad Complutense de Madrid, 28040 SpainSearch for more papers by this author First published: 31 March 2000 https://doi.org/10.1002/(SICI)1096-9861(20000417)419:4 3.0.CO;2-MCitations: 34Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The localization of nitrergic cells and fibers and cholinergic cells has been analyzed in the spinal cord of the anuran amphibian Rana perezi. Histochemistry for nicotinamide adenine dinucleotide phosphate–diaphorase and nitric oxide synthase immunohistochemistry revealed a concurrent pattern of labeled structures. A large population of nitrergic spinal neurons was found from the level of the obex to the filum terminale. They are abundant in the dorsal horn and intermediate gray matter, but also occur in territories of the ventral horn and, only occasionally, in somatic motoneurons. Numerous nitrergic fibers were present in the spinal white matter, particularly in the dorsal and dorsolateral funiculi. A special arrangement of nitrergic axons is present in Lissauer's tract, where a collateral system is formed. Cholinergic cells, revealed by choline acetyltransferase immunohistochemistry, were observed throughout the spinal cord. The somatic motoneurons were the most conspicuously immunoreactive cells. A large population of cholinergic cells forms a discontinuous column in the intermediate gray, from the third spinal segment to lumbar segments. These cells were organized in a medially located or intercalated cell group, and a laterally located intermediolateral group. Numerous scattered cholinergic cells were present in the central zone of the ventral horn and were absent in the dorsal horn. Double-labeling experiments revealed a high degree of codistribution of nitrergic and cholinergic cells, mainly in the intermediate gray, but colocalization of both markers in the same neurons was not found. 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