Radioimmunologic characterization of RD-114 reverse transcriptase: Evolutionary relatedness of mammalian type C viral pol gene products
1978; Elsevier BV; Volume: 86; Issue: 1 Linguagem: Inglês
10.1016/0042-6822(78)90013-2
ISSN1096-0341
AutoresJeffery M. Krakower, Stuart A. Aaronson,
Tópico(s)Hepatitis B Virus Studies
ResumoThe reverse transcriptase of RD-114, an endogenous mammalian type C virus, has been purified and characterized by radioimmunologic techniques. Using radioimmunoprecipitation assays, specific antibodies to reverse transcriptase were detected in the sera of animals naturally or experimentally infected with mammalian type C viruses. Competition radioimmunoassays using the purified enzyme as a probe detected type-specific antigenic determinants of RD-114 reverse transcriptase, as well as group-specific determinants shared with baboon endogenous virus. The pol gene products of all mammalian type C viruses were shown to share interspecies antigenic determinants not detected among functionally analogous reverse transcriptases of other retroviruses. These findings, together with previous immunologic evidence, strongly favor a common progenitor in the evolution of mammalian type C viruses.
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