A study of enzyme-catalyzed product deposition on planar gold electrodes using electrical impedance measurement
1995; Wiley; Volume: 7; Issue: 3 Linguagem: Inglês
10.1002/elan.1140070313
ISSN1521-4109
AutoresD. Athey, M Ball, Calum J. McNeil, R.D. Armstrong,
Tópico(s)Electrochemical sensors and biosensors
ResumoElectroanalysisVolume 7, Issue 3 p. 270-273 Feature Article A study of enzyme-catalyzed product deposition on planar gold electrodes using electrical impedance measurement Dale Athey, Corresponding Author Dale Athey Cambridge Life Sciences plc, Cambridgeshire Business Park, Angel Drove, Ely, CB7 4DT, UKCambridge Life Sciences plc, Cambridgeshire Business Park, Angel Drove, Ely, CB7 4DT, UKSearch for more papers by this authorMark Ball, Mark Ball Department of Clinical Biochemistry, The Medical School, University of Newcastle Upon Tyne, NE2 4HH, UKSearch for more papers by this authorCalum J. McNeil, Calum J. McNeil Department of Clinical Biochemistry, The Medical School, University of Newcastle Upon Tyne, NE2 4HH, UKSearch for more papers by this authorRon D. Armstrong, Ron D. Armstrong Department of Chemistry, Bedson Building, University of Newcastle Upon Tyne, NE1 7RU, UKSearch for more papers by this author Dale Athey, Corresponding Author Dale Athey Cambridge Life Sciences plc, Cambridgeshire Business Park, Angel Drove, Ely, CB7 4DT, UKCambridge Life Sciences plc, Cambridgeshire Business Park, Angel Drove, Ely, CB7 4DT, UKSearch for more papers by this authorMark Ball, Mark Ball Department of Clinical Biochemistry, The Medical School, University of Newcastle Upon Tyne, NE2 4HH, UKSearch for more papers by this authorCalum J. McNeil, Calum J. McNeil Department of Clinical Biochemistry, The Medical School, University of Newcastle Upon Tyne, NE2 4HH, UKSearch for more papers by this authorRon D. Armstrong, Ron D. Armstrong Department of Chemistry, Bedson Building, University of Newcastle Upon Tyne, NE1 7RU, UKSearch for more papers by this author First published: March 1995 https://doi.org/10.1002/elan.1140070313Citations: 32AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract An electrical impedance based specific binding assay which utilizes the avidin-biotin interaction is described. The specific binding of a biotinylated enzyme label, alkaline phosphatase, is determined by monitoring the change in capacitance of an avidin-coated planar gold electrode. The enzyme label alkaline phosphatase converts the substrate 5-bromo-4-chloro-3-indolyl phosphate to an insoluble dimeric product which adheres specifically to the surface of the gold electrode and causes a maximum decrease in electrode capacitance of 55%. This capacitance change is directly related to the amount of biotinylated enzyme bound to the surface of the electrode. Electrical impedance analysis has been used to characterize the surface effects on the electrode. Citing Literature Volume7, Issue3March 1995Pages 270-273 RelatedInformation
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