Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Microbial enantioselective reduction of ethyl-2-oxo-4-phenyl-butanoate

2006; Elsevier BV; Volume: 28; Issue: 3 Linguagem: Inglês

10.1016/j.bej.2006.01.003

1873-295X

Paulo Sérgio Bergo de Lacerda, Joyce Benzaquem Ribeiro, Selma Gomes Ferreira Leite, Ricardo B. Coelho, Edson Luiz da Silva Lima, Octávio Augusto Ceva Antunes,

Amino Acid Enzymes and Metabolism

Different microorganisms (MOs) were used to carry out the enantioselective reduction of ethyl-2-oxo-4-phenylbutanoate to (S)-(+)-2-hydroxy-4-phenylbutanoate or (R)-(+)-2-hydroxy-4-phenylbutanoate. Commercially available Saccharomyces cerevisiae and Dekera sp. led to over 92% ee of (S)-(+)-2-hydroxy-4-phenylbutanoate. Kluyveromyces marxianus gave the opposite isomer with 32% ee (R). All reactions, except those with Hansenula sp., proceeded to greater than 90% conversion. This the first report on the use of Dekera sp., Hansenula sp. and K. marxianus in the reduction of α-ketoesters.