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Cyclization of squalene-2,3-oxide to lanosterol in a cell-free system from Phycomyces blakesleeanus

1969; Elsevier BV; Volume: 8; Issue: 12 Linguagem: Inglês

10.1016/s0031-9422(00)88150-9

1873-3700

E.I. Mercer, Meredith Johnson,

Microbial Metabolic Engineering and Bioproduction

[14C]squalene was formed biosynthetically from [2-14C]mevalonic acid by a rat-liver enzyme preparation and converted via its 2,3-monobromohydrin into [14C]squalene-2,3-oxide. A cell-free enzyme preparation from Phycomyces blakesleeanus is described which catalysed the cyclization of the [14C]squalene-2,3-oxide to [14C]lanosterol.