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Inhibition of DNA synthesis by diflubenzuron in pupae of the stable fly Stomoxys calcitrans (L.)

1981; Elsevier BV; Volume: 15; Issue: 2 Linguagem: Inglês

10.1016/0048-3575(81)90083-3

1095-9939

John R. DeLoach, Shirlee M. Meola, Richard T. Mayer, James M. Thompson,

Insect and Pesticide Research

Biochemical assays of stable fly pupae treated with diflubenzuron at the white prepupal stage and then injected with either [3H]thymidine or [14C]thymidine showed no differences in uptake of the thymidine at 0–4, 0–24, 20–21, or 22–24 hr after injection of the radiolabeled thymidine. However, at 32–36 hr the diflubenzuron pupae incorporated only 10–11% of the amount of labeled thymidine incorporated by the untreated pupae. Autoradiographs taken from diflubenzuron-treated pupae at 22–24 and 32–36 hr after injection of the labeled thymidine showed that a reduction in DNA synthesis had occurred in cells originating from the imaginal epidermal histoblasts. The reduction in DNA synthesis at 22–24 hr was not detectable by the biochemical assay since the number of proliferating epidermal cells was too small a proportion of the total number of cells undergoing histogenesis at this time period. Thus, the insect growth regulator, diflubenzuron, appears to be cell specific in this species of fly in that the reduction in DNA synthesis is observed only in cells originating from the imaginal epidermal histoblasts. However, it is not known at this time whether this effect is primary or secondary.