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Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante

2007; Brazilian Chemical Society; Volume: 30; Issue: 7 Linguagem: Inglês

10.1590/s0100-40422007000700019

ISSN

1678-7064

Autores

Isis Martins Figueiredo, Anita J. Marsaioli,

Tópico(s)

Advanced MRI Techniques and Applications

Resumo

H NMR TECHNIQUES FOR DETECTING THE LIGAND.NMR is a valuable screening tool for the binding of ligands to proteins providing structural information on both protein and ligands and is thus largely applied to drug-discovery.Among the recent NMR techniques to probe weak binding protein-ligand complexes we have critically evaluated the advantages and disadvantages of STD (Saturation Transfer Difference), WaterLOGSY (Water Ligand Observation with Gradient Spectroscopy), NOE pumping and DOSY-NOESY (Diffusion-Ordered NOESY) using a mixture of BSA (bovine serum albumin) plus salicylic acid, caffeine, citric acid, adipic acid and D-glucose.

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