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Crystallization and preliminary X-ray diffraction analysis of two pH-dependent forms of a di-haem cytochrome c peroxidase from Pseudomonas nautica

2002; Wiley; Volume: 58; Issue: 4 Linguagem: Inglês

10.1107/s0907444902002354

1399-0047

João M.L. Dias, C. Bonifácio, T. Alves, José J. G. Moura, Isabel Moura, Maria João Romão,

Microbial Fuel Cells and Bioremediation

Two crystal forms of cytochrome c peroxidase from Pseudomonas nautica were obtained, one at pH 4.0 using sodium citrate as precipitant and another at pH 5.3 using ammonium phosphate and sodium citrate as precipitants. The two forms belong to different space groups P3121 (pH 4.0) and P6422 (pH 5.3), with unit-cell parameters a = b = 114.5, c = 90.7 Å and a = b = 151.0, c = 155.9 Å, respectively. Several complete data sets were collected using synchrotron radiation at ESRF and Cu Kα X-ray radiation from a rotating-anode generator. These results will contribute to clarifying the haem transitions occurring during peroxidatic reaction and the required electron-transfer processes and to elucidating the catalytic mechanism of the enzyme and the role of calcium in the activation process.