Efficient blue conversion from a 1064 nm microchip laser in long photonic crystal fiber tapers for fluorescence microscopy
2010; Optica Publishing Group; Volume: 18; Issue: 16 Linguagem: Inglês
10.1364/oe.18.016640
ISSN1094-4087
AutoresAlexandre Kudlinski, Mickaël Lelek, B. Barviau, Laurent Audry, Arnaud Mussot,
Tópico(s)Advanced Fluorescence Microscopy Techniques
ResumoUsing a low-cost microchip laser and a long photonic crystal fiber taper, we report a supercontinuum source with a very efficient visible conversion, especially in the blue region (around 420 nm). About 30 % of the total average output power is located in the 350-600 nm band, which is of primary importance in a number of biophotonics applications such as flow cytometry or fluorescence imaging microscopy for instance. We successfully demonstrate the use of this visible-enhanced source for a three-color imaging of HeLa cells in wide-field microscopy.
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