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A model system to study antimicrobial strategies in endodontic biofilms

2009; UNIVERSIDADE DE SÃO PAULO; Volume: 17; Issue: 2 Linguagem: Inglês

10.1590/s1678-77572009000200003

1678-7765

Carlos Estrela, Gilson Blitzkow Sydney, José Antônio Poli de Figueiredo, Cyntia Rodrigues de Araújo Estrela,

Dental Radiography and Imaging

The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.