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Pleiotropic Effects of Pasteurella multocida Toxin Are Mediated by Gq-dependent and -independent Mechanisms

2001; Elsevier BV; Volume: 276; Issue: 6 Linguagem: Inglês

10.1074/jbc.m007819200

1083-351X

Alexandra Zywietz, Antje Gohla, Milena Schmelz, Günter Schultz, Stefan Offermanns,

Rabies epidemiology and control

Pasteurella multocida toxin (PMT) is a highly potent mitogen for a variety of cell types. PMT has been shown to induce various cellular signaling processes, and it has been suggested to function through the heterotrimeric G-proteins G q /G 11 . To analyze the role of G q /G 11 in the action of PMT, we have studied the effect of the toxin in Gα q /Gα 11 double-deficient fibroblasts as well as in fibroblasts lacking only Gα q or Gα 11 . Interestingly, formation of inositol phosphates in response to PMT was exclusively dependent on Gα q but not on the closely related Gα 11 . Although Gα q /Gα 11 double-deficient and Gα q -deficient cells did not respond with any production of inositol phosphates to PMT, PMT was still able to induce various other cellular effects in these cells, including the activation of Rho, the Rho-dependent formation of actin stress fibers and focal adhesions, as well as the stimulation of c-Jun N-terminal kinase and extracellular signal-regulated kinase. These data show that PMT leads to a variety of cellular effects that are mediated only in part by the heterotrimeric G-protein G q .