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Distribution of glucokinase, glucose transporter GLUT2, sulfonylurea receptor-1, glucagon-like peptide-1 receptor and neuropeptide Y messenger RNAs in rat brain by quantitative real time RT-PCR

2003; Elsevier BV; Volume: 113; Issue: 1-2 Linguagem: Inglês

10.1016/s0169-328x(03)00125-6

1872-6941

Bing Li, Xiaochun Xi, David S. Roane, Donna H. Ryan, Roy J. Martin,

Biochemical Analysis and Sensing Techniques

Glucokinase (GK), glucose transporter GLUT2, sulfonylurea receptor-1 (SUR1), glucagon-like peptide-1 receptor (GLP-1R) and neuropeptide Y (NPY) have been proposed to be involved in central glucose sensing or regulation of food intake. In this study, we combined tissue micropunch and real time reverse transcription polymerase chain reaction (RT-PCR), and measured GK, GLUT2, SUR1, GLP-1R and NPY mRNA expression in discrete areas in the hypothalamus and the hindbrain.