Produção Nacional
Revisado por pares
Polymorphism in the Alpha Cardiac Muscle Actin 1 Gene Is Associated to Susceptibility to Chronic Inflammatory Cardiomyopathy
2013; Public Library of Science; Volume: 8; Issue: 12 Linguagem: Inglês
10.1371/journal.pone.0083446
ISSN1932-6203
AutoresAmanda Farage Frade, Priscila Camillo Teixeira, Bárbara Maria Ianni, Cristina Wide Pissetti, Bruno Saba, Hui Tzu Lin‐Wang, Andréia Kuramoto, Luciana Nogueira, Paula Buck, Fabrício C. Dias, Helene Giniaux, Agnès Llored, Sthefanny Alves, André Schmidt, Eduardo Antônio Donadi, José Antônio Marin‐Neto, Mário Hiroyuki Hirata, Marcelo Sampaio, Abílio Fragata, Edimar Alcides Bocchi, Antonio Noedir Stolf, Alfredo Inácio Fiorelli, Ronaldo Honorato Barros Santos, Virmondes Rodrigues, Alexandre C. Pereira, Jorge Kalil, Edécio Cunha‐Neto, Christophe Chevillard,
Tópico(s)Viral Infections and Immunology Research
ResumoAimsChagas disease, caused by the protozoan Trypanosoma cruzi is endemic in Latin America, and may lead to a life-threatening inflammatory dilated, chronic Chagas cardiomyopathy (CCC). One third of T. cruzi-infected individuals progress to CCC while the others remain asymptomatic (ASY). A possible genetic component to disease progression was suggested by familial aggregation of cases and the association of markers of innate and adaptive immunity genes with CCC development. Since mutations in multiple sarcomeric genes, including alpha-cardiac actin (ACTC1) have been involved in hereditary dilated cardiomyopathy, we investigated the involvement of the ACTC1 gene in CCC pathogenesis. Methods and ResultsWe conducted a proteomic and genetic study on a Brazilian study population. The genetic study was done on a main cohort including 118 seropositive asymptomatic subjects and 315 cases and the replication was done on 36 asymptomatic and 102 CCC cases. ACTC1 protein and mRNA levels were lower in myocardial tissue from patients with end-stage CCC than those found in hearts from organ donors. Genotyping a case-control cohort of CCC and ASY subjects for all informative single nucleotide polymorphism (SNP) in the ACTC1 gene identified rs640249 SNP, located at the 5' region, as associated to CCC. Associations are borderline after correction for multiple testing. Correlation and haplotype analysis led to the identification of a susceptibility haplotype. Functional assays have shown that the rs640249A/C polymorphism affects the binding of transcriptional factors in the promoter regions of the ACTC1 gene. Confirmation of the detected association on a larger independent replication cohort will be useful. ConclusionsGenetic variations at the ACTC1 gene may contribute to progression to chronic Chagas Cardiomyopathy among T. cruzi-infected patients, possibly by modulating transcription factor binding to ACTC1 promoter regions.
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