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Characterization of Soluble Non-covalent Complexes between Bovine Serum Albumin and β-1,2,3,4,6-Penta- O -galloyl- d -glucopyranose by MALDI-TOF MS

2004; American Chemical Society; Volume: 52; Issue: 12 Linguagem: Inglês

10.1021/jf035536t

1520-5118

Yumin Chen, Ann Hagerman,

Analytical Chemistry and Chromatography

Beta-1,2,3,4,6-penta-O-galloyl-D-glucopyranose (PGG) and soluble complexes of PGG with bovine serum albumin (BSA) were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). PGG was also characterized by electrospray ionization mass spectrometry (ESI-MS). Similar fragmentation patterns of PGG were found in ESI-MS and MALDI-TOF MS. The apparent stoichiometries of non-covalent BSA-PGG complexes were determined by MALDI-TOF MS.