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Karyotypic characterization of the bat species Molossus ater, M. molossus and Molossops planirostris (Chiroptera, Molossidae) using FISH and banding techniques

2003; BioMed Central; Volume: 138; Issue: 2 Linguagem: Inglês

10.1034/j.1601-5223.2003.01693.x

1601-5223

Cleide Leite-Silva, Neide Santos, Valéria Fagundes, Yatiyo Yonenaga-Yassuda, Maria José de Souza,

Genomics and Phylogenetic Studies

The karyotypes of the bat species Molossus ater, M. molossus (2n=48; NF=64) and Molossops planirostris (2n=34; NF=60) were analyzed by G-, C-banding, silver nitrate staining (AgNO3), base-specific fluorochromes and fluorescent in situ hybridization (FISH). The two species of Molossus presented the constitutive heterochromatin (CH) in the pericentromeric regions of all autosomes and in the X chromosome, while the Y chromosome was completely heterochromatic. Molossops planirostris showed conspicuous CH blocks in the pericentromeric regions of the pairs 4, 5, 8, 15, 16 and in the short arm of the X chromosome, while the Y did not present any CH block. Pretreated slides for C-banding stained with DAPI (CB-DAPI) revealed a similar pattern of C-banding (CBG) for these species. Sequential staining (AgNO3/CMA3/DAPI) in M. planirostris showed that the nucleolus organizer regions (NORs) are weakly CMA3 positive and DAPI negative. In the three species, triple staining with CMA3/DA/DAPI revealed R-banding with CMA3 and uniform staining with DAPI. The ribosomal cistrons detected by FISH were present only in the pair 5 in both species of Molossus, and in two pairs of medium sized chromosomes (pairs 9 and 10) of Molossops planirostris. The results obtained by FISH, compared with those by AgNO3 staining, indicated that all NORs in these species are transcriptionally active.