Portal de Periódicos da CAPES

Isolation, Structure Characterization, and Immunomodulating Activity of a Hyperbranched Polysaccharide from the Fruiting Bodies of Ganoderma sinense

2012; American Chemical Society; Volume: 60; Issue: 17 Linguagem: Inglês

10.1021/jf205056u

1520-5118

Xiaoqiang Han, Ben Chung-Lap Chan, Caixia Dong, Yongqiang Yang, Chun‐Hay Ko, Grace Gar‐Lee Yue, Dan Chen, Chris K.C. Wong, Clara Bik‐San Lau, Peng‐Fei Tu, Pang‐Chui Shaw, Kwok‐Pui Fung, Ping‐Chung Leung, Wen‐Luan Wendy Hsiao, Quan‐Bin Han,

Seaweed-derived Bioactive Compounds

A polysaccharide (GSP-6B) with a molecular mass of 1.86 × 106 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d-glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β-d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.