Mucopolysaccharide-degrading enzymes from the liver of the squid, Ommastrephes sloani pacificus I. Hyaluronidase
1971; Elsevier BV; Volume: 242; Issue: 2 Linguagem: Inglês
10.1016/0005-2744(71)90234-8
ISSN1878-1454
Autores Tópico(s)Polyamine Metabolism and Applications
Resumo1. Liver extracts of the squid, Ommastrephes sloani pacificus, contain mucopolysaccharide-degrading enzymes: hyaluronidase (hyaluronate glycanohydrolase, EC 3.2.1.35), β-N-acetylhexosaminidase (EC 3.2.1.30); β-glucuronidase (EC 3.2.1.31) and chondrosulphatase (EC 3.1.6.4). 2. The squid hyaluronidase which was partially purified by gel chromatography on Sephadex G-150 showed the maximum activity at pH 4.5–5.0 and the temperature of 37°. The activity was completely inhibited by Cu2+ and Fe3+. 3. Hyaluronate was the best substrate of the squid hyaluronidase and several sulphated mucopolysaccharides were also degraded. The digestion products of hyaluronate were identical with those obtained by bovine testicular hyaluronidase digestion, indicating that the squid liver hyaluronidase is an endo-β-N-aacetylhexosaminidase. 4. This seems to be the first example of a testicular type hyaluronidase found in invertebrate tissues.
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