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Inhibition of polyamine synthesis by cyclohexylamine stimulates the ethylene pathway and accelerates the germination of Cicer arietinum seeds

1994; Wiley; Volume: 91; Issue: 1 Linguagem: Inglês

10.1111/j.1399-3054.1994.tb00652.x

1399-3054

Mercedes Gallardo, Manuel Eduardo Gallardo, Ángel J. Matilla, P. Muñoz de Rueda, Isabel María Sánchez‐Calle,

Plant tissue culture and regeneration

Treatment with cyclohexylamine, an inhibitor of spermidine synthase, accelerated radicle emergence in chick‐pea ( Cicer arietinum L. cv. Castellana) seeds. Stimulation in the growth of embryonic axis was correlated with: (a) a rise in putrescine (Put) and cadaverine (Cad); (b) a decrease in spermidine (Spd) and spermine (Spm), and (c) a concomitant acceleration of the transformation from S‐adenosylmethionine (SAM) into ethylene with increases in the levels of l‐aminocyclopropane‐l‐carboxylic acid (ACC), l‐(malonylamino)cyclopropane‐l‐carboxylic acid (mACC) and ethylene and ACC synthase and ethylene‐forming enzyme activities. Cyclohexylamine also stimulated the mitotic index in both apical and subapical zones of the radicle and the apical zone of the plumule. The sectional distribution of the ethylene pathway and polyamine content was studied in embryonic axes of seeds germinated for 65 h. Each axis was divided into 5 sections: radicle meristem, elongation zone, differentiation zone, hypocotyl and plumule. In the presence of cyclohexylamine, ACC synthase and ACC were strongly stimulated in both the differentiation and hypocotyl zones, whereas the mACC was stimulated in all sections of the embryonic axis. With respect to ethylene‐forming enzyme activity and ethylene production, the hypocotyl and the zones of elongation and differentiation were affected most by cyclohexylamine. Cyclohexylamine also induced an accumulation of free Put and Spm in the differentiation, hypocotyl and radicle zones, whereas Put and Spm bound to small substances increased most in the hypocotyl and plumule. The Spd bound to small substances decreased in all sections in the presence of cyclohexylamine. With respect to polyamines bound to macromolecules, cyclohexylamine stimulated only the accumulation of Put since Spd and Spm were strongly inhibited in all sections.