Portal de Periódicos da CAPES

Chemical and pharmacological evaluation of fractions potentially active obtained of the aqueous extract of Capraria biflora L.

2010; Thieme Medical Publishers (Germany); Volume: 76; Issue: 12 Linguagem: Inglês

10.1055/s-0030-1264403

1439-0221

I Vicet, David Marrupe, D Siverio, María Elena Maldonado Celis, Peter de Witte, A.D. Crawford, Wim Dehaen, Luc Pieters, A Cuellar, Gustavo Sierra, Hoang Khanh Nguyen, Arthur W. Campbell,

Natural product bioactivities and synthesis

Capraria biflora L. is a wild plant very used in traditional medicine in American continent and in the Caribbean area (1). This plant is active as analgesic, diuretic and anti-inflammatory, equivalent to the reference drugs according to studies developed previously (2,3,4) but no detailed chemical study had been reported. The present work had as objective to identify the active fractions obtained from the aqueous extract of the leaves of this species and to deepen in the chemical composition of the active fractions. Aqueous extract was partitioned successively with chloroform and butanol to give the chloroform, butanol and water fractions, which were evaluated as anti-inflammatory using zebras fish like pharmacological model (5). The chloroform and butanol fraction was found the most active. The combination of thin layer chromatography and column chromatography and the spectroscopic evaluation of the isolated compounds allowed suggesting the presence of naringenin, mannitol, derived of apigenin and luteolin in the most active fractions. The identified compounds are informed for the first time in the plant, and they allow justifying the anti-inflammatory effects and diuretics shown by the aqueous extract of this species and the uses in traditional medicine.