Generation of defective interfering particles in picornaviruses
1980; Elsevier BV; Volume: 100; Issue: 2 Linguagem: Inglês
10.1016/0042-6822(80)90532-2
ISSN1096-0341
AutoresMarcella A. McClure, John J. Holland, Jacques Perrault,
Tópico(s)Animal Virus Infections Studies
ResumoWe have extensively searched for defective interfering (DI) particles in several picornaviruses. Contrary to expectations, only the originally described poliovirus type I strain (C. N. Cole, D. Smoller, E. Wimmer, and D. Baltimore, 1971, J. Virol.7, 478–485) gave rise to DI separable in CsCl density gradients. However, by using high resolution gel analysis of glyoxal-denaturated RNAs, we detected small deletions (4–6%) in the virus particle genomes from long-term undiluted passage of mengovirus and two independent strains of poliovirus type I. Virus stocks enriched for these smaller RNAs were shown to interfere with the replication of standard virus. DI generation appears to be strain dependent since a third isolate of poliovirus type I does not give rise to detectable genome deletions even after 100 passages. Furthermore, a cloned stock of the Cole et al. strain regenerates a low density DI as observed originally. We also present evidence suggesting that host cell factors modulate both generation and interference level of a picornavirus DI (mengovirus). Vaccine reference strains of poliovirus did not contain detectable deleted genomes in contrast to a preliminary report from this laboratory. These studies have important implications for the possible role of DI in human or other picornavirus infections.
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