Effects of VIP, TRH, GABA and dopamine on prolactin release from superfused rat anterior pituitary cells
1983; Elsevier BV; Volume: 32; Issue: 11 Linguagem: Inglês
10.1016/0024-3205(83)90196-0
ISSN1879-0631
AutoresNorio Matsushita, Yuzuru Kato, Akira Shimatsu, Hideki Katakami, Noboru Yanaihara, Hiroo Imura,
Tópico(s)Receptor Mechanisms and Signaling
ResumoEffects of VIP, TRH, dopamine and GABA on the secretion of prolactin (PRL) from rat pituitary cells were studied in vitro with a sensitive superfusion method. Dispersed anterior pituitary cells were placed on a Sephadex G-25 column and continuously eluted with KRBG buffer. Infusion of TRH (10−11−10−8M) and VIP (10−9−10−6M) resulted in a dose-related increase in PRL release. LHRH (10−8−10−5M) had no effect on PRL release. On the other hand, infusion of dopamine (10−9−10−6M) and GABA (10−8−10−4M) suppressed not only the basal PRL release from dispersed pituitary cells but also the PRL response to TRH and VIP. The potency of TRH to stimulate PRL release is greater than that of VIP, and the potency of dopamine to inhibit PRL secretion is stronger than that of GABA on a molar basis. These results indicate that TRH and VIP have a stimulating role whereas dopamine and GABA have an inhibitory role in the regulation of PRL secretion at the pituitary level in the rat.
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