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Envelope Glycoproteins of HIV-1, HIV-2, and SIV Purified with Galanthus nivalis Agglutinin Induce Strong Immune Responses

1993; Mary Ann Liebert, Inc.; Volume: 9; Issue: 5 Linguagem: Inglês

10.1089/aid.1993.9.431

1931-8405

Gustav Gilljam,

Carbohydrate Chemistry and Synthesis

Lectin affinity chromatography was used to purify in a single step the envelope glycoproteins of HIV-1, HIV-2, and SIV. Envelope glycoproteins carry the major determinants essential for protection by the humoral immune response. The purification of these proteins has previously been a laborious procedure. The glycoproteins were purified by a one-step procedure to a high level of purity by using Galanthus nivalis agglutinin (GNA). The purified glycoprotein had CD4-binding and antigenic reactivities. Strong immune responses to envelope proteins and peptides were seen in mice and primates after immunization with these preparations.