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The crystal structure of putative precorrin isomerase CbiC in cobalamin biosynthesis

2005; Elsevier BV; Volume: 153; Issue: 3 Linguagem: Inglês

10.1016/j.jsb.2005.11.011

1095-8657

Yanyan Xue, Zhiyi Wei, Xu Li, Weimin Gong,

Folate and B Vitamins Research

The leptospira cbiC encodes the enzyme catalyzing the methyl rearrangement reaction of the cobalamin biosynthesis pathway. The protein has been cloned and overexpressed as a His-tagged recombinant protein in Escherichia coli. The crystal structures have been solved in two crystal forms (P42212 and P3121) diffracting to 3.0 and 2.3 Å resolution, respectively. The structures are similar to the precorrin-8x methyl mutase (CobH), an enzyme of the aerobic pathway to vitamin B12.