Promoter elements required for sugar‐repression of the RAmy3D gene for α‐amylase in rice
1998; Wiley; Volume: 428; Issue: 3 Linguagem: Inglês
10.1016/s0014-5793(98)00518-3
ISSN1873-3468
AutoresKyoko Toyofuku, Taka-aki Umemura, Junji Yamaguchi,
Tópico(s)Plant tissue culture and regeneration
ResumoThere is increasing evidence showing that cereal alpha-amylase gene expression is controlled not only by the classical hormonal regulation, but also by feed-back sugar repression. We demonstrated by in situ hybridization that the sugar repression of rice alpha-amylase gene RAmy3D takes place in scutellar epithelium cells of callus-forming rice embryos. We also used a transient expression system to study the cis-acting elements involved in the sugar repression of the RAmy3D promoter activity. Site-directed mutagenesis of the 50-bp nucleotide sequence from -172 to -123 revealed that consensus sequences of G motif (TACGTA) and TATCCA T/C motif (GATA motif as its antisense sequence) are responsible for sugar repression. The promoter sequences required for sugar repression are reported and discussed.
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