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Fabrication of Biofunctionalized Quasi-Three-Dimensional Microstructures of a Nonfouling Comb Polymer Using Soft Lithography

2005; Wiley; Volume: 15; Issue: 4 Linguagem: Inglês

10.1002/adfm.200400088

1616-3028

Hui‐Lin Ma, Jinho Hyun, Z. Zhang, Thomas P. Beebe, Ashutosh Chilkoti,

Cellular Mechanics and Interactions

Advanced Functional MaterialsVolume 15, Issue 4 p. 529-540 Full Paper Fabrication of Biofunctionalized Quasi-Three-Dimensional Microstructures of a Nonfouling Comb Polymer Using Soft Lithography† H. Ma, H. Ma Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708-0281, USASearch for more papers by this authorJ. Hyun, J. Hyun School of Biological Resources and Materials Engineering, Seoul National University, Seoul, 151-742, Korea Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708-0281, USASearch for more papers by this authorZ. Zhang, Z. Zhang Department of Chemistry & Biochemistry, University of Delaware, Newark, DE19716, USASearch for more papers by this authorT. P. Beebe Jr., T. P. Beebe Jr. Department of Chemistry & Biochemistry, University of Delaware, Newark, DE19716, USASearch for more papers by this authorA. Chilkoti, A. Chilkoti chilkoti@duke.edu Search for more papers by this author H. Ma, H. Ma Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708-0281, USASearch for more papers by this authorJ. Hyun, J. Hyun School of Biological Resources and Materials Engineering, Seoul National University, Seoul, 151-742, Korea Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708-0281, USASearch for more papers by this authorZ. Zhang, Z. Zhang Department of Chemistry & Biochemistry, University of Delaware, Newark, DE19716, USASearch for more papers by this authorT. P. Beebe Jr., T. P. Beebe Jr. Department of Chemistry & Biochemistry, University of Delaware, Newark, DE19716, USASearch for more papers by this authorA. Chilkoti, A. Chilkoti chilkoti@duke.edu Search for more papers by this author First published: 23 March 2005 https://doi.org/10.1002/adfm.200400088Citations: 39 † The authors thank Mr. Yong Wang for help with the viscosity measurements. This work was partially supported by the Center for Biologically Inspired Materials and Materials System (CBIMMS) at Duke University and by the NSF through grant EEC-0210590 to A. C. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract This paper describes a simple set of patterning methods that are applicable to diverse substrates and allow the routine and rapid fabrication of protein patterns embedded within a background that consists of quasi-three-dimensional microstructures of a cell-resistant polymer. The ensemble of methods reported here utilizes three components to create topographically nonfouling polymeric structures that present cell-adhesive protein patterns in the regions between the microstructures: the first component is an amphiphilic comb polymer that is comprised of a methyl methacrylate backbone and pendant oligo(ethylene glycol) moieties along the side chain, physically deposited films of which are protein- and cell-resistant. The second component of the fabrication methodology involves the use of different variants of soft lithography, such as microcontact printing to create nonfouling topographical features of the comb polymer that demarcate cell-adhesive regions of the third component: a cell-adhesive extracellular protein or peptide. The ensemble of methods reported in this paper was used to fabricate quasi-three-dimensional patterns that present topographical and biochemical cues on a variety of substrates, and was shown to successfully maintain cellular patterns for up to two months in serum-containing medium. We believe that this, and other such methods under development that allow independent and systematic control of chemistry, topography and substrate compliance will provide versatile “test-beds” for fundamental studies in cell biology as well as allow the discovery of rational design principles for the development of biomaterials and tissue-engineering scaffolds. Citing Literature Volume15, Issue4April, 2005Pages 529-540 RelatedInformation