Stimulation of in vitro shoot organogenesis in Glycine max (Merrill.) by allantoin and amides
1992; Elsevier BV; Volume: 81; Issue: 2 Linguagem: Inglês
10.1016/0168-9452(92)90048-q
ISSN1873-2259
AutoresKalidas Shetty, Yasuhiro Asano, Katsuji Oosawa,
Tópico(s)Seed Germination and Physiology
ResumoEfficient de novo shoot organogenesis, directly from cut quadrant explants of the epicotyl-hypocotyl area (near the cotyledonary nodal region) of Glycine max (soybean), was stimulated by organic products of biological nitrogen fixation (amides and the ureide, allantoin) in the presence of benzyladenine. These organic nitrogen compounds had a stimulatory effect on shoot organogenesis only when the inorganic nitrogen source, ammonium nitrate was lowered to 120 of the level present in standard Murashige and Skoog (MS) medium. All other nitrogen combinations resulted in poor multiple shoot formation and in many cases enhanced callus formation. Ureide in the form of allantoin and amides (glutamine and asparagine) were critical components which enhanced shoot organogenesis and reduced callus formation.
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