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Artigo Produção Nacional Revisado por pares
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Spontaneous Effects of Low-Level Laser Therapy (650 nm) in Acute Inflammatory Mouse Pleurisy Induced by Carrageenan

2005; Mary Ann Liebert, Inc.; Volume: 23; Issue: 4 Linguagem: Inglês

10.1089/pho.2005.23.377

ISSN

1557-8550

Autores

Rodrigo Álvaro Brandão Lopes-Martins, Regiane Albertini, Patrícia Sardinha Leonardo Lopes, Jan Magnus Bjordal, Hugo C. Castro Faria Neto,

Tópico(s)

Marine Invertebrate Physiology and Ecology

Resumo

Objective: Our aim was to investigate the effect of low-level laser therapy (LLLT), 650-nm wavelength, on acute inflammatory pleurisy. Background data: There is only scattered evidence of anti-inflammatory effects from LLLT and dosage characteristics, and the effect on pleurisy inflammation has yet to be investigated. Methods: A classical experimental model of pleurisy was used in a sample of 40 Balb male mice, randomly divided into five groups. Inflammation was induced by carrageenan (0.5 mg/cavity) administered by intrathoracic injections. Four groups received the inflammatory agent, and one received injections of sterile saline solution. At 1, 2, and 3 h after injections, LLLT irradiation was performed, with the same power (2.5 mW), but different irradiation times. The energy densities at each of the three treatment sessions were 0 J/cm2 (placebo), 3 J/cm2, 7.5 J/cm2, and 15 J/cm2, respectively. Results: Total and differential cell analysis at 4 h after induction of pleurisy showed a significant reduction of inflammatory cell migration for all groups treated with active laser. However, at 4 h after injection, the most significant (p < 0.001) reduction of leukocyte cell migration was seen in the 7.5 J/cm2 group, at 2.7 (95% CI: 2.5–2.9) × 106, versus 7.9 (95% CI: 6.7–9.1) × 106 in the placebo control group. The greatest reduction of inflammatory cells was registered for neutrophils. Conclusions: LLLT administered at 1–3 h after the induction of inflammatory pleurisy significantly reduces the inflammatory cell migration measured. Under these conditions and at 2.5 mW, 7.5 J/cm2 was more effective than 3 J/cm2 and 15 J/cm2.

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