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A comment on the design of experiments to estimate the Michaelis-Menten parameters of enzyme-catalysed reactions

1981; Birkhäuser; Volume: 37; Issue: 2 Linguagem: Inglês

10.1007/bf01963182

0014-4754

Gordon L. Atkins, I.A. Nimmo,

Microbial Metabolic Engineering and Bioproduction

Michaelis-Menten parameters can be estimated by measuring initial velocities (v) in replicate at 2 concentrations of substrate, one much lower than Km and the other much higher than it. Analysis of simulated experimental data suggests that this design will probably give more precise estimates of Km and V than the conventional design in which v is measured at several different concentrations of substrate.