Characterization of microbial mutagens in complex samples—methodology and application
1984; CRC Press; Volume: 14; Issue: 2 Linguagem: Inglês
10.1080/10643388409381715
ISSN2374-9733
AutoresIngrid Alfheim, Alf Bjørseth, Mona Møller, Gerald L. Fisher,
Tópico(s)Pharmaceutical and Antibiotic Environmental Impacts
ResumoMethodologies for characterization of mutagens in complex mixtures have been reviewed. The methods discussed include successive fractionation with various solvents, combinations of chemical fractionation and bio‐assay directed analyses and separation with in situ testing using thin layer chromatography or other separation techniques. In all cases the discussions are limited to the Ames Salmonella assay as detection method for mutagenicity. Particular problems in testing complex samples such as bacterial toxicity, synergistic or antagonistic effects are also discussed. Applications of the methods are reviewed. These applications include environmental samples such as airborne particles, water pollutants, and consumer products.
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