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Preconditioning with glycyrrhizic, ferulic, paeoniflorin, cinnamic prevents rat hearts from ischemia/reperfusion injury via endothelial nitric oxide pathway

2015; SAGE Publishing; Volume: 11; Issue: 42 Linguagem: Inglês

10.4103/0973-1296.153081

0976-4062

Guo-Ping Zhao, Guo-Qiang Qian, Jingjing Ding, Xiaozhao Zhang, Xiaofeng Yin, Yuqin Gao,

Cardiac Ischemia and Reperfusion

Pharmacognosy Magazine,2015,11,42,292-296.DOI:10.4103/0973-1296.153081Published:March 2015Type:Original ArticleAuthors:Guo-Qiang Qian, Jingjing Ding, Xiaozhao Zhang, Xiaofeng Yin, Yuqin Gao, and Guo-Ping Zhao Author(s) affiliations:Guo-Qiang Qian1, Jingjing Ding1, Xiaozhao Zhang1, Xiaofeng Yin1, Yuqin Gao1, Guo-Ping Zhao2 1 Department of Nursing Science, Huanghuai University, Zhumadian, China 2 Department of Traditional Chinese Medicine, Medical College, Jinan University, Guangzhou, China Abstract:Objective: The objective was to investigate the endothelial nitric oxide synthase (eNOS/NO) pathway is involved or not in the protective effects of glycyrrhizic, ferulic, paeoniflorin, cinnamic (GFPC) in myocardial ischemia-reperfusion injury Sprague-Dawley rats. Materials and Methods: Ischemia-reperfusion (I/R) model was made by ligating the left anterior descending branch of the coronary artery for 30 min and releasing for 120 min, then the left ventricular apical was fixed and sliced, morphological changes of myocardial microvascular endothelial cell (MMVEC) was observed by electron microscopy, apoptosis index of MMVEC was observed by means of TUNEL, serum NO was tested by methods of nitrate reduction, lactate dehydrogenase (LDH), creatine kinase MB (CK-MB) was detected by automatic biochemical analyzer; Phosphorylated eNOS (PeNOS) and inducible NOS (iNOS) protein were measured by means of western blot. Results: In positive product control group, the serum levels of NO, LDH, CK-MB significantly increased (P < 0.05); MMVEC apoptosis was significantly decreased (P < 0.05); incidence of area at risk decreased significantly (P < 0.05); PeNOS protein increased (P < 0.05); iNOS protein decreased significantly (P < 0.05). Conclusion: Ischemic preconditioning of GFPC from GFPC plays a protective role in I/R heart through regulating the eNOS/NO signal pathway by increasing the PeNOS protein expression and decreasing the expression of iNOS protein. Keywords:Cinnamic, endothelial nitric oxide synthasee, ferulic, glycyrrhizic, inducible nitric oxide synthase, myocardial ischemia- reperfusion injury, myocardial microvascular endothelial cell, paeoniflorinView:PDF (1.64 MB) Full Text