Mitochondria hyperpolarization is an early event in oxidized low‐density lipoprotein‐induced apoptosis in Caco‐2 intestinal cells
2002; Wiley; Volume: 523; Issue: 1-3 Linguagem: Inglês
10.1016/s0014-5793(02)02972-1
ISSN1873-3468
AutoresClaudio Giovannini, Paola Matarrese, Beatrice Scazzocchio, Massimo Sanchez, Roberta Masella, Walter Malorni,
Tópico(s)ATP Synthase and ATPases Research
ResumoWe investigated the mechanisms underlying the pro-apoptotic activity exerted by oxidized low-density lipoproteins (oxLDL) in Caco-2 intestinal cells, a cell line which retains many morphological and enzymatic features typical of normal human enterocytes. We found that: (i) oxLDL induced mitochondrial-mediated apoptosis by provoking first an increase in mitochondrial membrane potential, followed, later, by the typical apoptosis-associated depolarization (type II apoptosis); accordingly, (ii) caspase-9 inhibition significantly hindered apoptosis while caspase-8 inhibition did not; and finally (iii) dietary phenolic antioxidizing compounds exerted a significant protective antiapoptotic activity. These results point to mitochondrial hyperpolarization as 'sensitizing feature' in apoptotic proneness of Caco-2 intestinal cells to oxLDL exposure.
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