Solid-state UV laser-induced fluorescence detection in capillary electrophoresis
2000; Wiley; Volume: 21; Issue: 10 Linguagem: Inglês
10.1002/1522-2683(20000601)21
ISSN1522-2683
AutoresKing C. Chan, Gary M. Muschik, Haleem J. Issaq,
Tópico(s)Electrowetting and Microfluidic Technologies
ResumoTwo solid-state UV lasers were applied to the laser-induced fluorescence (LIF) detection of various groups of compounds after separation by capillary electrophoresis. These lasers are thermoelectric-cooled, highly compact, and inexpensive. Such lasers provide few mW of quasi-continuous wave (CW) power which are sufficient and stable for LIF detection. Native fluorescence detection of tryptophan-containing proteins and peptides and related indoles was achieved at the nM level with the laser operating at 266 nm. Detection of fluorescamine-labeled amino acids and peptides was also possible at the nM level with the laser operating at 355 nm. Amino acids at a concentration as low as 10 ng/mL could be labeled with fluorescamine. Solid-state UV-LIF detection of the tryptic digest of cytochrome c after fluorescamine derivatization was demonstrated.
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