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Changes in gene expression profile in two multidrug resistant cell lines derived from a same drug sensitive cell line

2014; Elsevier BV; Volume: 38; Issue: 8 Linguagem: Inglês

10.1016/j.leukres.2014.06.001

1873-5835

Miguel Ângelo Martins Moreira, Carolina Bagni, Marcos Barcelos de Pinho, Thaís Messias Mac-Cormick, Mateus dos Santos Mota, Flávio Eduardo Pinto-Silva, Nathalia Daflon-Yunes, Vivian M. Rumjanek,

Epigenetics and DNA Methylation

Resistance to chemotherapy is one of the most relevant aspects of treatment failure in cancer. Cell lines are used as models to study resistance. We analyzed the transcriptional profile of two multidrug resistant (MDR) cell lines (Lucena 1 and FEPS) derived from the same drug-sensitive cell K562. Microarray data identified 130 differentially expressed genes (DEG) between K562 vs. Lucena 1, 1932 between K562 vs. FEPS, and 1211 between Lucena 1 versus FEPS. The NOTCH pathway was affected in FEPS with overexpression of NOTCH2 and HEY1. The highly overexpressed gene in MDR cell lines was ABCB1, and both presented the ABCB1 promoter unmethylated.