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Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates: a model for the interconversion of d-[2-3H]glucose 6-phosphate and d-[1-3H]fructose 6-phosphate

1990; Elsevier BV; Volume: 72; Issue: 4 Linguagem: Inglês

10.1016/0300-9084(90)90080-z

1638-6183

Veronique Liemans, Hakan Alp Bodur, F Malaisse-Lagae, Willy Malaisse,

Pancreatic function and diabetes

Based on experimental data, a model is proposed for the interconversion of either unlabelled hexose phosphates or d-[2-3H]glucose 6-phosphate and d-[1-3H]fructose 6-phosphate in the reaction catalyzed by phosphoglucoisomerase. This model takes into account the known differences in maximal velocity and affinity for each substrate, the intramolecular transfer of tritium between C1 and C2, and the isotopic discrimination between unlabelled and tritiated esters. This model revelas that, in a close system characterized by the progressive detritiation of hexose phosphates, the concentration ratio of d-glucose 6-phosphate is much higher with the tritiated than unlabelled esters, a paradoxical increase in the specific radioactivity of d-glucose 6-phosphate above its initial value being even observed during the initial period of exposure of d-[2-3H]glucose 6-phosphate to phosphoglucoisomerase. The extension of this model to an open system may be essential for the correct interpretation of radioactive data collected in intact cells exposed to d-[2-3H]glucose.