Portal de Periódicos da CAPES

Effect of Aldosterone and Glycyrrhetinic Acid on the Protein Expression of PAI-1 and p22phox in Human Mononuclear Leukocytes

2004; Oxford University Press; Volume: 89; Issue: 4 Linguagem: Inglês

10.1210/jc.2003-031545

1945-7197

Lorenzo A. Calò, Francesca Zaghetto, Elisa Pagnin, Paul A. Davis, Paola de Mozzi, Paola Sartorato, Giuseppe Martire, Cristina Fiore, Decio Armanini,

Pharmacological Effects of Natural Compounds

Abstract Aldosterone excess can produce heart and kidney fibrosis, which seem to be related to a direct effect of aldosterone at the level of specific receptors. We report a direct, mineralocorticoid-mediated effect on the protein expression of two markers of oxidative stress after incubation of mononuclear leukocytes with 1 × 10−8m aldosterone (p22phox/β-actin = 1.38 ± 0.05 and PAI-1/β-actin = 1.80 ± 0.05). The same effect was also found with 3 × 10−5m glycyrrhetinic acid, the principal constituent of licorice root (p22phox/β-actin = 1.37 ± 0.97 and PAI-1/β-actin = 1.80 ± 0.04). The effect of both aldosterone and glycyrrhetinic acid is blocked by incubation with added 1 × 10−6m of receptor-antagonist canrenone. Canrenone alone did not show any effect. PAI-1 related protein was also found using 4 × 10−9m aldosterone. Incubations with 1 × 10−9m for 3 hours as well as 1 × 10−8m aldosterone for 5, 10, and 20 minutes were ineffective for both proteins. These data support the previous finding of an involvement of mononuclear leukocytes in the pathogenesis of the oxidative stress induced by hyperaldosteronism. In addition, the results confirm our previous data on a direct effect of glycyrrhetinic acid at the level of mineralocorticoid receptors.