Cu I ‐semiquinone radical species in plant copper‐amine oxidases
1999; Wiley; Volume: 453; Issue: 1-2 Linguagem: Inglês
10.1016/s0014-5793(99)00675-4
ISSN1873-3468
AutoresRosaria Medda, Alessandra Padiglia, Andrea Bellelli, Jens Z. Pedersen, Alessandro Finazzi Agrò, Giovanni Floris,
Tópico(s)Porphyrin Metabolism and Disorders
ResumoThe intermediate Cu I ‐semiquinone radical species in the catalytic mechanism of copper‐amine oxidase from Lens esculenta and Pisum sativum seedlings has been studied by optical, Raman resonance and ESR spectroscopies and by stopped‐flow and temperature‐jump measurements. Treatment of highly purified enzyme preparations with good, poor or suicide substrates, under anaerobic and aerobic conditions, at different pH values and temperatures, makes it possible to generate, detect and characterize this free radical intermediate.
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