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Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection

2000; Wiley; Volume: 61; Issue: 2 Linguagem: Inglês

10.1002/(sici)1096-9071(200006)61

1096-9071

Shin‐Ru Shih, Yi-Shuane Li, Chiuan-Chian Chiou, Pin-Chau Suen, Tzou‐Yien Lin, Luan‐Yin Chang, Yhu‐Chering Huang, Kuo‐Chien Tsao, Hsiao-Chen Ning, Tzong-Zeng Wu, Err–Cheng Chan,

Immunodeficiency and Autoimmune Disorders

Journal of Medical VirologyVolume 61, Issue 2 p. 228-234 Expression of caspid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection Shin-Ru Shih, Shin-Ru Shih School of Medical Technology, Chang Gung University, TaiwanSearch for more papers by this authorYi-Shuane Li, Yi-Shuane Li School of Medical Technology, Chang Gung University, TaiwanSearch for more papers by this authorChiuan-Chian Chiou, Chiuan-Chian Chiou School of Medical Technology, Chang Gung University, TaiwanSearch for more papers by this authorPin-Chau Suen, Pin-Chau Suen Department of Clinical Pathology, and Department of Dentistry, Chang Gung Memorial Hospital, Taoyuan, TaiwanSearch for more papers by this authorTzou-Yien Lin, Tzou-Yien Lin Department of Infectious Diseases, Chang Gung Children's Hospital, TaiwanSearch for more papers by this authorLuan-Yin Chang, Luan-Yin Chang Department of Infectious Diseases, Chang Gung Children's Hospital, TaiwanSearch for more papers by this authorYhu-Chering Huang, Yhu-Chering Huang Department of Infectious Diseases, Chang Gung Children's Hospital, TaiwanSearch for more papers by this authorKuo-Chien Tsao, Kuo-Chien Tsao Clinical Virology Laboratory, Institute of Biotechnology, TaiwanSearch for more papers by this authorHsiao-Chen Ning, Hsiao-Chen Ning Clinical Virology Laboratory, Institute of Biotechnology, TaiwanSearch for more papers by this authorTzong-Zeng Wu, Tzong-Zeng Wu Institute of Biotechnology, National Dong Hwa University, TaiwanSearch for more papers by this authorErr-Cheng Chan, Corresponding Author Err-Cheng Chan [email protected] School of Medical Technology, Chang Gung University, TaiwanSchool of Medical Technology, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Taoyuan 333, Taiwan===Search for more papers by this author Shin-Ru Shih, Shin-Ru Shih School of Medical Technology, Chang Gung University, TaiwanSearch for more papers by this authorYi-Shuane Li, Yi-Shuane Li School of Medical Technology, Chang Gung University, TaiwanSearch for more papers by this authorChiuan-Chian Chiou, Chiuan-Chian Chiou School of Medical Technology, Chang Gung University, TaiwanSearch for more papers by this authorPin-Chau Suen, Pin-Chau Suen Department of Clinical Pathology, and Department of Dentistry, Chang Gung Memorial Hospital, Taoyuan, TaiwanSearch for more papers by this authorTzou-Yien Lin, Tzou-Yien Lin Department of Infectious Diseases, Chang Gung Children's Hospital, TaiwanSearch for more papers by this authorLuan-Yin Chang, Luan-Yin Chang Department of Infectious Diseases, Chang Gung Children's Hospital, TaiwanSearch for more papers by this authorYhu-Chering Huang, Yhu-Chering Huang Department of Infectious Diseases, Chang Gung Children's Hospital, TaiwanSearch for more papers by this authorKuo-Chien Tsao, Kuo-Chien Tsao Clinical Virology Laboratory, Institute of Biotechnology, TaiwanSearch for more papers by this authorHsiao-Chen Ning, Hsiao-Chen Ning Clinical Virology Laboratory, Institute of Biotechnology, TaiwanSearch for more papers by this authorTzong-Zeng Wu, Tzong-Zeng Wu Institute of Biotechnology, National Dong Hwa University, TaiwanSearch for more papers by this authorErr-Cheng Chan, Corresponding Author Err-Cheng Chan [email protected] School of Medical Technology, Chang Gung University, TaiwanSchool of Medical Technology, Chang Gung University, 259 Wen-Hua 1st Road, Kwei-Shan, Taoyuan 333, Taiwan===Search for more papers by this author First published: 28 April 2000 https://doi.org/10.1002/(SICI)1096-9071(200006)61:2 3.0.CO;2-RCitations: 29AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract To produce enterovirus 71 antigen for diagnostic purposes, the gene encoding the entire capsid protein VP1 was amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned and expressed in Escherichia coli as a poly-histidine fusion protein. Western blotting experiments with sera from patients with enterovirus 71 infection indicated that immunoglobulin G (IgG) and IgM antibodies bound to a single polypeptide VP1. According to these results, IgM anti-VP1 appeared in sera of patients with a symptomatic enterovirus 71 acute infection, whereas IgG anti-VP1 was present in sera of past infection. This finding suggests that detecting IgG and IgM immune responses against linear epitopes of recombinant VP1 is an effective means of determining the different phases of enterovirus 71 infection. In addition, sera containing coxsackie virus 16 (CA16) antibodies did not cross-react with the recombinant VP1 of enterovirus 71, despite the homology between VP1 proteins of both viruses. Comparison with reference PCR and neutralization assays showed these antibody tests to be appropriate for the serodiagnosis of enterovirus 71 infection. J. Med. Virol. 61:228–234, 2000. © 2000 Wiley-Liss, Inc. REFERENCES Blomberg J, Lycke E, Ahlfors K, Johnson T, Wolontis S, von Zeipel G. 1974. New enterovirus type associated with epidemic of aseptic meningitis or hand, foot, and mouth disease. Lancet 2: 112. Chang LY, Huang YC, Lin ZY. 1998. Fulminant neurogenic pulmonary edema with hand, foot, and mouth disease. Lancet 352: 367. Chomczynski P, Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156– 159. Chonmaitree T, Menegus MA, Schervish-Swierkosz EM, Schwalenstocker E. 1981. Enterovirus 71 infection: report of an outbreak with two cases of paralysis and a review of the literature. Pediatrics 67: 489– 493. Gilbert GL, Kickson EE, Waters MJ, Kennett ML, Land SA, Sneddon M. 1988. Outbreak of enterovirus 71 infection in Victoria, Australia, with a high incidence of neurologic involvement. Ped Infect Dis 8: 611– 616. Hagiwara A, Tagaya I, Yoneyama T. 1978. Epidemic of hand, foot and mouth disease associated with enterovirus type 71 infection. Inter Virol 9: 60– 63. Hagiwara A, Yoneyama T, Takami S, Hashimoto I. 1984. Tenotic and phenotypic characteristics of enterovirus 71 isolates from patients with encephalitis and with hand, foot and mouth disease. Arch Virol 79: 273– 283. Ishimaru Y, Nakano S, Yamaoka K, Takami S. 1980. Outbreaks of hand, foot, and mouth disease by enterovirus 71. Arch Dis Child 55: 583– 588. Kennett M, Birch CJ, Lewis FA, Yung AP, Locarnini SA, Gust ID. 1974. Enterovirus type 71 infection in Melbourne. Bull WHO 51: 609– 615. Lin YL, Liu JS, Chen KT, Chen CT, Chan EC. 1998. Identification of neutral and acidic sphingomyelinase in Helicobacter pylori. FEBS Lett 423: 249– 253. Lowe PE, Rhind SK, Sugrue R, Marston FAO. 1987. Solubilization, refolding and purification of eukaryotic proteins expressed in E. coli. In: R Alan, editor. Protein purification, micro to macro. New York: Liss Inc. p 429– 442. Melnick JL. 1984. Enterovirus type 71 infections: a varied clinical patients sometimes mimicking paralytic poliomyelitis. Rev Infect Dis 6: 387– 390. Nagy G, Takatsy S, Kukan E, Mihaly I, Domok I. 1982. Virological diagnosis of enterovirus type 71 infections: experiences gained during an epidemic of acute CNS diseases in Hungary in 1978. Arch Virol 71: 217– 227. Rosenberg AH, Lade BN, Chui DS, Lin SW, Dunn JJ, Studier FW. 1987. Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56: 125– 135. Rueckert RR. 1990. Picornaviruses and their replication. In: BN Fields, DM Knipe, editors. Virology. New York: Lippincott-Raven. p 507. Samuda GM, Chang WK, Yeung CY, Tang PS. 1987. Monoplegia caused by enterovirus 71: an outbreak in Hong Kong. Ped Infect Dis 6: 206– 208. Schmidt NJ, Lennette EH, Ho HH. 1974. An apparently new enterovirus isolated from patients with disease of the central nervous system. J Infect Dis 129: 304– 309. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Meth Enzymol 185: 60– 89. World Health Organization. 1988. Procedure for using the lyophilized LBM pools for typing enterovirus. Geneva: WHO. Zheng ZM, He PJ, Caueffield D, Neumann M, Specter S, Baker CC, Bankowski MJ. 1995. Enterovirus 71 isolated from China is serologically similar to the prototype E71 BrCr strain but differs in the 5′-noncoding region. J Med Virol 47: 161– 167. Citing Literature Volume61, Issue2June 2000Pages 228-234 ReferencesRelatedInformation