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Live pigs produced from genome edited zygotes

2013; Nature Portfolio; Volume: 3; Issue: 1 Linguagem: Inglês

10.1038/srep02847

2045-2322

Simon Lillico, Chris Proudfoot, Daniel F. Carlson, Dana Štveráková, C. Neil, Carol Blain, Tim King, William A. Ritchie, Wenfang Tan, Alan J. Mileham, David G. McLaren, Scott C. Fahrenkrug, Bruce Whitelaw,

Virus-based gene therapy research

Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.