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Fluorescence quenching immunoassay of serum cortisol

1980; Elsevier BV; Volume: 36; Issue: 2 Linguagem: Inglês

10.1016/0039-128x(80)90017-3

1878-5867

Kobayashi Yoshiharu, Tsubota Noriko, K Miyai, Fukuko Watanabe,

thermodynamics and calorimetric analyses

Fluorescence quenching immunoassay of serum cortisol was established. The minimal amount of cortisol detected was 3.1 ng/tube and serum concentration of 3.1 μg/dl to 100 μg/dl of cortisol could be measured. Intra- and inter-assay coefficient of variation were 7.7–10.5% and 10.7–13.3%, respectively. Cortisol values determined by the present method correlated well with those determined by radioimmunoassay (r = 0.97, y = 0.89 x + 0.88, n = 33). This fluorescence quenching immuno-assay satisfied the standard criteria of dilution and accuracy, and is a rapid and simple method requiring no antibody-bound and free separation.