Specific quantitation of plasma dehydroepiandrosterone-sulphate by GC-MS: Comparison with a direct RIA
1980; Elsevier BV; Volume: 106; Issue: 1 Linguagem: Inglês
10.1016/0009-8981(80)90379-4
ISSN1873-3492
AutoresD. W. Johnson, G. Phillipou, S K James, C J Seaborn, M. M. Ralph,
Tópico(s)Pesticide Residue Analysis and Safety
ResumoDehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEAS) are endogenous steroids that have recently been widely publicized as potential treatments for many disorders. This paper describes a gas chromatographic–ion trap mass spectrophotometric assay with selected reaction monitoring for measurement of DHEA and DHEAS levels. The hormones and internal standard (5-androsten-3β-ol-16-one methyl ester) are extracted from serum with Oasis solid-phase extraction tubes. The extracted steroids are dissolved in methanol and injected into a Finnigan GCQ ion trap mass spectrometer. In the selected reaction mode, both DHEA and DHEAS can be identified and quantified in a single injection. No derivatization or expensive deuterated internal standards are required.
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