A candidate interferon-gamma activated site (GAS element) in the HLA-G promoter does not bind nuclear proteins
1999; Elsevier BV; Volume: 60; Issue: 11 Linguagem: Inglês
10.1016/s0198-8859(99)00091-9
ISSN1879-1166
AutoresWenjiang Chu, Jianjun Gao, William J. Murphy, Joan S. Hunt,
Tópico(s)Cytokine Signaling Pathways and Interactions
ResumoThe HLA-G gene is highly expressed at the maternal-fetal interface, where it is believed to participate in the generation and maintenance of maternal tolerance to the fetal semiallograft. This gene has two elements through which interferon-gamma (IFN-gamma) could act to enhance its rate of transcription, an Enhancer A/ICS region and a candidate IFN-gamma activated site (GAS). In this study we investigated functionality of this candidate HLA-G GAS. Two HLA-G-expressing cell lines were tested, the human myelomonocytic cell line, U937, and a mouse fibroblast cell line, S14/8, which is stably transfected with the full length HLA-G gene. Nuclear proteins from IFN-gamma-treated U937 and S14/8 cells bound the interferon regulatory factor-1 (IRF-1) gene GAS sequence (TTC CCCGAA) but not the HLA-G gene's candidate GAS sequence (TTTCGAGAA). Excess unlabeled HLA-G-GAS oligonucleotide failed to inhibit binding of the IRF-1-GAS using the same nuclear extracts. These data indicate that a sequence in the HLA-G gene which would normally permit cytokine enhancement of gene expression, the GAS element, is nonfunctional. This is also true of another regulatory sequence, the Enhancer A/ICS element, suggesting that defects in IFN-gamma response elements prevent inappropriate up-regulation of HLA-G gene expression at the maternal-fetal interface.
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