A 46-nucleotide promoter segment from an IFN-α gene renders an unrelated promoter inducible by virus
1985; Cell Press; Volume: 41; Issue: 2 Linguagem: Inglês
10.1016/s0092-8674(85)80023-4
ISSN1097-4172
AutoresJohn Ryals, Peter M. Dierks, Hermann Ragg, C WEISSMANN,
Tópico(s)RNA and protein synthesis mechanisms
ResumoSummary A hybrid gene consisting of the human IFN-α1 promoter and a β-globin transcription unit is expressed correctly only after viral induction. To determine the region required for inducibility, 25 hybrid promoters consisting of varying upstream IFN-α1 and downstream β-globin promoter moieties were analyzed, and 5′-deletion analysis was performed on an inducible hybrid promoter. An IFN promoter region from position −109 to −64 conferred maximal inducibility on downstream β-globin promoter segments and even on the intact β-globin promoter. This region is strikingly conserved among human IFN-α and -β genes. As constitutive expression of the β-globin gene was not diminished by placing IFN promoter fragments in various positions, induction is attributed largely to positive, rather than to negative control.
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