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X.—MICROSCOPICAL DETERMINATIONS OF THE THICKNESS OF HISTOLOGICAL SECTIONS

1954; Royal Microscopical Society; Volume: 74; Issue: 2 Linguagem: Inglês

10.1111/j.1365-2818.1954.tb02009.x

1747-342X

Sven‐Olof Brattgård,

Image Processing Techniques and Applications

SYNOPSIS A theoretical analysis is made of determinations of the thickness of specimens in the microscope, based on Abbe and Berek's formulae. The accuracy of focusing has been stated to amount to one‐fifth of the vertical resolving power. Making full use of the optical system in a Leitz Ortholux microscope, it is calculated to be < ± 0.1μ. The theoretical calculations are verified experimentally. The standard error of the determination of the thickness in the microscope is found experimentally to amount to ±0.16μ,. With a measured thickness of 5μ, the error is thus ±3.2 p.c. Definitions are given of the following terms: depth of focus, depth of field, vertical resolving power, depth of vision, and accuracy of focusing.