Portal de Periódicos da CAPES

Arterial heparan sulfate proteoglycans inhibit vascular smooth muscle cell proliferation and phenotype change in vitro and neointimal formation in vivo

1998; Elsevier BV; Volume: 28; Issue: 2 Linguagem: Inglês

10.1016/s0741-5214(98)70167-3

1097-6809

John Bingley, Ian P. Hayward, Julie H. Campbell, Gordon Campbell,

Cardiac Valve Diseases and Treatments

Abstract Purpose: The aim of this study was to determine whether heparan sulfate proteoglycans (HSPGs) from the normal arterial wall inhibit neointimal formation after injury in vivo and smooth muscle cell (SMC) phenotype change and proliferation in vitro. Methods: Arterial HSPGs were extracted from rabbit aortae and separated by anion-exchange chromatography. The effect of HSPGs, applied in a periadventitial gel, on neointimal formation was assessed 14 days after balloon catheter injury of rabbit carotid arteries. Their effect on SMC phenotype and proliferation was measured by point-counting morphometry of the cytoplasmic volume fraction of myofilaments (Vvmyo) and 3 H-thymidine incorporation in SMCs in culture. Results: Arterial HSPGs (680 μg) reduced neointimal formation by 35% at 14 days after injury ( P = .029), whereas 2000 μg of the low-molecular-weight heparin Enoxaparin was ineffective. HSPGs at 34 μg/mL maintained subconfluent primary cultured SMCs with the same high Vvmyo (52.1% ± 13.8%) after 5 days in culture as did cells freshly isolated from the arterial wall (52.1% ± 15.1%). In contrast, 100 μg/mL Enoxaparin was ineffective in preventing phenotypic change over this time period (Vvmyo 38.9% ± 14.6%, controls 35.9% ± 12.8%). HSPGs also inhibited 3 H-thymidine incorporation into primary cultured SMCs with an ID 50 value of 0.4 μg/mL compared with a value of 14 μg/mL for Enoxaparin ( P < .01). Conclusion: When used periadventitially in the rabbit arterial injury model, natural arterial HSPGs are effective inhibitors of neointimal formation. In vitro, the HSPGs maintain SMCs in a quiescent state by inhibiting phenotypic change and DNA synthesis. This study suggests that HSPGs may be a natural agent for the treatment of clinical restenosis. (J Vasc Surg 1998;28:308-18)