Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein
1981; Elsevier BV; Volume: 116; Issue: 1 Linguagem: Inglês
10.1016/0003-2697(81)90321-3
ISSN1096-0309
AutoresSteve M. Read, D. H. Northcote,
Tópico(s)Advanced Proteomics Techniques and Applications
ResumoModifications to the Coomassie blue G dye-binding assay for protein are described which remove much of the variation previously observed in the response of this assay to different proteins. Increasing the concentration of dye in the assay solution, or decreasing the concentration of phosphoric acid, increased the color yield of a range of proteins to values close to that of the standard protein bovine serum albumin. The sensitivity of the assay was also increased so that a protein concentration of 2 μg/ml can be accurately determined in a sample volume of 50 μl.
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